Production of polyclonal antibody against the recombinant PirB^vp protein of Vibrio parahaemolyticus.

ABSTRACT:

Background

Acute hepatopancreatic necrosis disease (AHPND) is caused by toxin-producing strains of Vibrio parahaemolyticus which contain deadly binary toxins PirA^vp and PirB^vp encoded in pVA1 plasmid. The polyclonal antibodies against PirB^vp protein could be used to develop immunochromatographic test strip for in-field diagnosis of AHPND.

Results

In this study, PirB^vp gene was amplified, cloned, and expressed in E. coli. The expressed protein was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot probed with 6xHis antibodies. Then, the recombinant PirB^vp (rPirB^vp) was purified using Ni-Sepharose column. Rabbits were immunized with the purified rPirB^vp,

SUBMITTER: Duong ND 

PROVIDER: S-EPMC8113428 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature