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Facile isolation and analysis of sporopollenin exine from bee pollen.

ABSTRACT: We present facile methods to obtain purified sporopollenin exine capsules, and provide mass balances for classical and novel purification procedures. An ionic liquid, tetrabutyl phosphonium hydroxide turned out to be the most effective in removing the intine wall. The sporopollenin capsules were investigated by fluorescent microscopy, AFM, solid-state NMR and infrared Raman spectroscopy. The latter two methods showed that sunflower and rape exines have different proportions of O-aliphatic and aromatic constituents. Purified exine capsules were coated with functionalized fluorophores. The procedures presented in this paper could contribute to further spread of the applications of this hollow, and chemically highly resistant material.

SUBMITTER: Hegedus K

PROVIDER: S-EPMC8113464 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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Facile isolation and analysis of sporopollenin exine from bee pollen.

Hegedüs Kristóf K Fehér Csaba C Jalsovszky István I Kristóf Zoltán Z Rohonczy János J Vass Elemér E Farkas Attila A Csizmadia Tamás T Friedbacher Gernot G Hantz Peter P

Scientific reports 20210511 1

We present facile methods to obtain purified sporopollenin exine capsules, and provide mass balances for classical and novel purification procedures. An ionic liquid, tetrabutyl phosphonium hydroxide turned out to be the most effective in removing the intine wall. The sporopollenin capsules were investigated by fluorescent microscopy, AFM, solid-state NMR and infrared Raman spectroscopy. The latter two methods showed that sunflower and rape exines have different proportions of O-aliphatic and ar ...[more]

PMID: 33976296

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