MYLK4 promotes tumor progression through the activation of epidermal growth factor receptor signaling in osteosarcoma.

ABSTRACT:

Background

Osteosarcoma (OS) is the most common primary bone cancer in adolescents and lung metastasis is the leading cause of death in patients with OS. However, the molecular mechanisms that promote OS growth and metastasis remain unknown.

Methods

We investigated the expression of myosin light chain kinase family members between metastasis and non-metastasis patients in the TARGET database and ensured that only myosin light chain kinase family member 4 (MYLK4) had higher expression in metastatic osteosarcoma patients. Then we confirmed the results by immunohistochemistry (IHC) and Western blotting (WB) of OS tissues. The effect of MYLK4 on the metastasis and proliferation of OS cells was investigated by wound healing, Transwell and colony-formation assays. Mass spectrum an

SUBMITTER: Yang M 

PROVIDER: S-EPMC8114533 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature