Ultralow amounts of DNA from long-term archived serum samples produce high-quality methylomes.

ABSTRACT:

Background

Long-term stored serum is considered challenging for epigenomic analyses: as there are no cells, circulating DNA is scarce, and amplification removes epigenetic signals. Additionally, pre-analytical treatments and storage might introduce biases and fragmentation to the DNA. In particular, starting with low-input DNA can result in low-diversity libraries. However, successful whole-genome bisulphite sequencing (WGBS) of such serum samples has the potential to open biobanks for epigenetic analyses and deliver novel prediagnostic biomarkers. Here, we perform WGBS using the Accel-NGS library preparation kit on ultralow amounts of DNA from long-term archived samples with diverse pretreatments from the Janus Serum Bank.

Results

Ninety-four of the 96 samples produced sati

SUBMITTER: Wojewodzic MW 

PROVIDER: S-EPMC8114536 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature