Thermostable Cellulase Biosynthesis from Paenibacillus alvei and its Utilization in Lactic Acid Production by Simultaneous Saccharification and Fermentation.

ABSTRACT: Cellulosic date palm wastes may have beneficial biotechnological applications for eco-friendly utilization. This study reports the isolation of thermophilic cellulase-producing bacteria and their application in lactic acid production using date palm leaves. The promising isolate was identified as Paenibacillus alvei by 16S rRNA gene sequencing. Maximum cellulase production was acquired using alkaline treated date palm leaves (ATDPL) at 48 h and yielded 4.50 U.mL^-1 FPase, 8.11 U.mL^-1 CMCase, and 2.74 U.mL^-1 β-glucosidase. The cellulase activity was optimal at pH 5.0 and 50°C with good stability at a wide temperature (40-70°C) and pH (4.0-7.0) range, demonstrating its suitability in simultaneous saccharification and fermentation. Lactic acid fermentati

SUBMITTER: Mostafa YS 

PROVIDER: S-EPMC8114780 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature