Engineered human myocardium with local release of angiogenic proteins improves vascularization and cardiac function in injured rat hearts.

ABSTRACT: Heart regeneration after myocardial infarction requires new cardiomyocytes and a supportive vascular network. Here, we evaluate the efficacy of localized delivery of angiogenic factors from biomaterials within the implanted muscle tissue to guide growth of a more dense, organized, and perfused vascular supply into implanted engineered human cardiac tissue on an ischemia/reperfusion injured rat heart. We use large, aligned 3-dimensional engineered tissue with cardiomyocytes derived from human induced pluripotent stem cells in a collagen matrix that contains dispersed alginate microspheres as local protein depots. Release of angiogenic growth factors VEGF and bFGF in combination with morphogen sonic hedgehog from the microspheres into the local microenvironment occurs from the epicardial imp

SUBMITTER: Munarin F 

PROVIDER: S-EPMC8115013 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature