Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae.

ABSTRACT: Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, multiphoton and light sheet microscopy. All these techniques suffer from anisotropic resolution and limited penetration depth. In the past, Multiview microscopy-imaging the sample from different angles followed by 3D image reconstruction-was developed to address this issue for light sheet microscopy based on fluorescence signal. In this study we applied this methodology to accomplish Multiview imaging with multiphoton microscopy based on fluorescence and additionally second harmonic signal from myosin and collagen. It was shown that isotropic resolution was achieved, the entirety of the sample was visualized, and interference arti

SUBMITTER: Kapsokalyvas D 

PROVIDER: S-EPMC8115086 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature