The m⁶A methylome of SARS-CoV-2 in host cells.

ABSTRACT: The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a global health emergency because of its rapid spread and high mortality. The molecular mechanism of interaction between host and viral genomic RNA is yet unclear. We demonstrate herein that SARS-CoV-2 genomic RNA, as well as the negative-sense RNA, is dynamically N⁶-methyladenosine (m⁶A)-modified in human and monkey cells. Combined RIP-seq and miCLIP analyses identified a total of 8 m⁶A sites at single-base resolution in the genome. Especially, epidemic strains with mutations at these identified m⁶A sites have emerged worldwide, and formed a unique cluster in the US as indicated by phylogenetic analysis. Further functional experiments showed that m<

SUBMITTER: Liu J 

PROVIDER: S-EPMC8115241 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature