Reduced intracellular chloride concentration impairs angiogenesis by inhibiting oxidative stress-mediated VEGFR2 activation.

ABSTRACT: Chloride (Cl^-) homeostasis is of great significance in cardiovascular system. Serum Cl^- level is inversely associated with the mortality of patients with heart failure. Considering the importance of angiogenesis in the progress of heart failure, this study aims to investigate whether and how reduced intracellular Cl^- concentration ([Cl^-]_i) affects angiogenesis. Human umbilical endothelial cells (HUVECs) were treated with normal Cl^- medium or low Cl^- medium. We showed that reduction of [Cl^-]_i (from 33.2 to 16.18 mM) inhibited HUVEC proliferation, migration, cytoskeleton reorganization, tube formation, and subsequently suppressed angiogenesis under basal condition, and VEGF stimulation or hypox

SUBMITTER: Li K 

PROVIDER: S-EPMC8115249 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature