Development of a multiplex mass spectrometry method for simultaneous quantification of urinary proteins related to respiratory health.

ABSTRACT: Respiratory health of children is a health priority. Club cell protein (CC16) is an interesting biomarker of lung diseases and adverse effects towards the airway epithelium integrity. Osteopontin (OPN) and nuclear factor-kappa B (NF-κB) also play a role in respiratory health. The use of urine as biomarker source is useful in studies involving children but necessitates proper adjustment for physiological confounders influencing the urinary excretion, potentially characterized with beta-2-microglobulin (β2M), retinol binding protein 4 (RBP4) or myoglobin (MYO), as well as adjustment for possible renal dysfunction, characterized by human serum albumin (HSA). The simultaneous quantification of all these proteins in urine could facilitate children's health monitoring. A multiple reaction monito

SUBMITTER: Nauwelaerts SJD 

PROVIDER: S-EPMC8115669 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature