Nucleic acid sample preparation from whole blood in a paper microfluidic device using isotachophoresis.

ABSTRACT: Nucleic acid amplification tests (NAATs) are a crucial diagnostic and monitoring tool for infectious diseases. A key procedural step for NAATs is sample preparation: separating and purifying target nucleic acids from crude biological samples prior to nucleic acid amplification and detection. Traditionally, sample preparation has been performed with liquid- or solid-phase extraction, both of which require multiple trained user steps and significant laboratory equipment. The challenges associated with sample preparation have limited the dissemination of NAAT point-of-care diagnostics in low resource environments, including low- and middle-income countries. We report on a paper-based device for purification of nucleic acids from whole blood using isotachophoresis (ITP) for point-of-care NAATs

SUBMITTER: Sullivan BP 

PROVIDER: S-EPMC8115986 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature