Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification.

ABSTRACT: A method for the simultaneous determination of seven B-group vitamers including thiamine, riboflavin, nicotinamide, niacin, pyridoxine, pyridoxal, and pyridoxamine in nutritional products by using enzymatic digestion followed by LC-MS/MS quantification was studied. The LC-MS/MS conditions such as MS transitions, mobile phase programs, and ammonium formate buffer concentrations, and sample treatment procedures (e.g., concentrations of buffer solution, digestion temperature, and digestion time) were investigated. The analytical method performance was evaluated by multiple criteria such as selectivity, linearity, detection and quantification limits, repeatability, reproducibility, and recovery by using real sample matrices. The validated method was successfully applied to analyze vitamin B co

SUBMITTER: Nguyen QH 

PROVIDER: S-EPMC8116160 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature