Evaluating the Consistency of Gene Methylation in Liver Cancer Using Bisulfite Sequencing Data.

ABSTRACT: Bisulfite sequencing is considered as the gold standard approach for measuring DNA methylation, which acts as a pivotal part in regulating a variety of biological processes without changes in DNA sequences. In this study, we introduced the most prevalent methods for processing bisulfite sequencing data and evaluated the consistency of the data acquired from different measurements in liver cancer. Firstly, we introduced three commonly used bisulfite sequencing assays, i.e., reduced-representation bisulfite sequencing (RRBS), whole-genome bisulfite sequencing (WGBS), and targeted bisulfite sequencing (targeted BS). Next, we discussed the principles and compared different methods for alignment, quality assessment, methylation level scoring, and differentially methylated region identification.

SUBMITTER: Zheng X 

PROVIDER: S-EPMC8116545 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature