METTL3 restrains papillary thyroid cancer progression via m⁶A/c-Rel/IL-8-mediated neutrophil infiltration.

ABSTRACT: Growing evidence indicates that N6-methyladenosine (m⁶A) is the most pervasive RNA modification in eukaryotic cells. However, the specific role of METTL3 in papillary thyroid carcinoma (PTC) initiation and development remains elusive. Here we found that downregulation of METTL3 was correlated with malignant progression and poor prognosis in PTC. A variety of gain- and loss-of-function studies clarified the effect of METTL3 on regulation of growth and metastasis of PTC cells in vitro and in vivo. By combining RNA sequencing (RNA-seq) and methylated RNA immunoprecipitation sequencing (meRIP-seq), our mechanistic studies pinpointed c-Rel and RelA as downstream m⁶A targets of METTL3. Disruption of METTL3 elicited secretion of interleukin-8 (IL-8), and elevated concentrati

SUBMITTER: He J 

PROVIDER: S-EPMC8116572 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature