Identification of Hub Genes and MicroRNAs Associated With Idiopathic Pulmonary Arterial Hypertension by Integrated Bioinformatics Analyses.

ABSTRACT:

Objective

The aim of this study is the identification of hub genes associated with idiopathic pulmonary arterial hypertension (IPAH).

Materials and methods

GSE15197 gene expression data was downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified by screening IPAH patients and controls. The 5,000 genes with the greatest variances were analyzed using a weighted gene co-expression network analysis (WGCNA). Modules with the strongest correlation with IPAH were chosen, followed by a functional enrichment analysis. Protein-protein interaction (PPI) networks were constructed to identify hub gene candidates using calculated degrees. Real hub genes were found from the overlap of DEGs and candidate hub genes. microRNAs (miRNAs)

SUBMITTER: Qiu X 

PROVIDER: S-EPMC8117102 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature