Vitamin D₃ suppresses intestinal epithelial stemness via ER stress induction in intestinal organoids.

ABSTRACT:

Background

Vitamin D₃ is important for normal function of the intestinal epithelial cells (IECs). In this study, we aimed to investigate the effects of vitamin D₃ on the differentiation, stemness, and viability of healthy IECs in intestinal organoids.

Methods

Intestinal organoids derived from mouse small intestine were treated with vitamin D₃, and the effects on intestinal stemness and differentiation were evaluated using real-time PCR and immunofluorescence staining of the distinct lineage markers. Cell viability was analyzed using viability and apoptosis assays.

Results

Vitamin D₃ enhanced IEC differentiation into the distinct lineages of specialized IECs, including Paneth, goblet, and enteroendocrine cells and absorptive

SUBMITTER: Sittipo P 

PROVIDER: S-EPMC8117327 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature