ATF5 and HIF1α cooperatively activate HIF1 signaling pathway in esophageal cancer.

ABSTRACT:

Background

Esophageal cancer (ESCA) is one of the most common cancers worldwide and has a very poor prognosis. Hypoxia-inducible factor 1 (HIF1) signaling pathway plays a critical role in tumorigenesis and is therefore considered a potential therapeutic target in the treatment of many cancers. Activating transcription factor 5 (ATF5) facilitates the expression of various genes and has been extensively studied for its potential role in cancer treatment.

Methods

The expression level of ATF5 in clinic sample was detected by quantitative real time PCR and immunohistochemistry. ATF5 biological function was investigated by western blot, cell cycle analysis, cell viability assay, luciferase reporter assays, colony formation assay, transwell assay, wound healing assay, tube formatio

SUBMITTER: He F 

PROVIDER: S-EPMC8117505 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature