Calcium-Regulated Protein CarP Responds to Multiple Host Signals and Mediates Regulation of Pseudomonas aeruginosa Virulence by Calcium.

ABSTRACT: Pseudomonas aeruginosa is an opportunistic pathogen causing life-threatening infections. Previously, we showed that elevated calcium (Ca²⁺) levels increase the production of virulence factors in P. aeruginosa In an effort to characterize the Ca²⁺ regulatory network, we identified a Ca²⁺-regulated β-propeller protein, CarP, and showed that expression of the encoding gene is controlled by the Ca²⁺-regulated two-component system CarSR. Here, by using a Galleria melonella model, we showed that CarP plays a role in regulating P. aeruginosa virulence. By using transcriptome sequencing (RNA-Seq), reverse transcription (RT)-PCR, quantitative RT-PCR (RT-qPCR), and promoter fusions, we determined that car

SUBMITTER: King M 

PROVIDER: S-EPMC8117776 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature