The impact of genetic background and sex on the phenotype of IL-23 induced murine spondyloarthritis.

ABSTRACT:

Background

Overexpression of IL-23 in adult mice by means of hydrodynamic tail vein injection of IL-23 minicircles has been reported to result in spondyloarthritis-like disease. The impact of genetic background and sex on the disease phenotype in this model has not been investigated.

Methods

We compared male B10.RIII mice with male C57BL/6 mice, and male with female B10.RIII mice after hydrodynamic injection of IL-23 enhanced episomal vector (EEV) at 8-12 weeks of age. We monitored clinical arthritis scores, paw swelling, and body weight. Animals were euthanized after two weeks and tissues were harvested for histology, flow cytometry and gene expression analysis. Serum cytokine levels were determined by ELISA.

Findings

Male B10.RIII mice developed arthritis in the for

SUBMITTER: Haley EK 

PROVIDER: S-EPMC8118278 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature