Mitochondrial Function and Anesthetic Sensitivity in the Mouse Spinal Cord.

ABSTRACT:

Background

Ndufs4 knockout (KO) mice are defective in mitochondrial complex I function and hypersensitive to inhibition of spinal cord-mediated response to noxious stimuli by volatile anesthetics. It was hypothesized that, compared to wild-type, synaptic or intrinsic neuronal function is hypersensitive to isoflurane in spinal cord slices from knockout mice.

Methods

Neurons from slices of the vestibular nucleus, central medial thalamus, and spinal cord from wild-type and the global Ndufs4 knockout were patch clamped. Unstimulated synaptic and intrinsic neuronal characteristics were measured in response to isoflurane. Norfluoxetine was used to block TREK channel conductance. Cholinergic cells were labeled with tdTomato.

Results

All values are reported as means and 95% C

SUBMITTER: Woods CB 

PROVIDER: S-EPMC8119385 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature