Faster monitoring of the invasive alien species (IAS) Dreissena polymorpha in river basins through isothermal amplification.

ABSTRACT: Zebra mussel (Dreissena polymorpha) is considered as one of the 100 most harmful IAS in the world. Traditional detection methods have limitations, and PCR based environmental DNA detection has provided interesting results for early warning. However, in the last years, the development of isothermal amplification methods has received increasing attention. Among them, loop-mediated isothermal amplification (LAMP) has several advantages, including its higher tolerance to the presence of inhibitors and the possibility of naked-eye detection, which enables and simplifies its potential use in decentralized settings. In the current study, a real-time LAMP (qLAMP) method for the detection of Dreissena polymorpha was developed and tested with samples from the Guadalquivir River basin, together with

SUBMITTER: Carvalho J 

PROVIDER: S-EPMC8119715 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature