Impact of Three Different Serum Sources on Functional Properties of Equine Mesenchymal Stromal Cells.

ABSTRACT: Culture and expansion of equine mesenchymal stromal cells (MSCs) are routinely performed using fetal bovine serum (FBS) as a source of growth factors, nutrients, and extracellular matrix proteins. However, the desire to minimize introduction of xenogeneic bovine proteins or pathogens and to standardize cellular products intended for clinical application has driven evaluation of alternatives to FBS. Replacement of FBS in culture for several days before administration has been proposed to reduce antigenicity and potentially prolong survival after injection. However, the functional consequences of MSC culture in different serum types have not been fully evaluated. The objective of this study was to compare the immunomodulatory and antibacterial properties of MSCs cultured in three serum sourc

SUBMITTER: Pezzanite L 

PROVIDER: S-EPMC8119767 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature