Temporal analysis of type 1 interferon activation in tumor cells following external beam radiotherapy or targeted radionuclide therapy.

ABSTRACT: Rationale: Clinical interest in combining targeted radionuclide therapies (TRT) with immunotherapies is growing. External beam radiation therapy (EBRT) activates a type 1 interferon (IFN1) response mediated via stimulator of interferon genes (STING), and this is critical to its therapeutic interaction with immune checkpoint blockade. However, little is known about the time course of IFN1 activation after EBRT or whether this may be induced by decay of a TRT source. Methods: We examined the IFN1 response and expression of immune susceptibility markers in B78 and B16 melanomas and MOC2 head and neck cancer murine models using qPCR and western blot. For TRT, we used ⁹⁰Y chelated to NM600, an alkylphosphocholine analog that exhibits selective uptake and retention in tu

SUBMITTER: Jagodinsky JC 

PROVIDER: S-EPMC8120207 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature