Heterologous Expression and Biochemical Characterization of the Human Zinc Transporter 1 (ZnT1) and Its Soluble C-Terminal Domain.

ABSTRACT: Human zinc transporter 1 (hZnT1) belongs to the cation diffusion facilitator (CDF) family. It plays a major role in transporting zinc (Zn²⁺) from the cytoplasm across the plasma membrane and into the extracellular space thereby protecting cells from Zn²⁺ toxicity. Through homology with other CDF family members, ZnT1 is predicted to contain a transmembrane region and a soluble C-terminal domain though little is known about its biochemistry. Here, we demonstrate that human ZnT1 and a variant can be produced by heterologous expression in Saccharomyces cerevisiae cells and purified in the presence of detergent and cholesteryl hemisuccinate. We show that the purified hZnT1 variant has Zn²⁺/H⁺ antiporter activity. Furthermore, we expressed, puri

SUBMITTER: Cotrim CA 

PROVIDER: S-EPMC8120272 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature