Expression of the qepA1 gene is induced under antibiotic exposure.

ABSTRACT:

Background

The qepA1 gene encodes an efflux pump that reduces susceptibility to ciprofloxacin. Little is known about the regulation of qepA1 expression.

Objectives

To assess the potential role of ciprofloxacin and other antibiotics in the regulation of qepA1 gene expression. To identify the promoter that drives qepA1 expression and other factors involved in expression regulation. To assess whether the identified features are universal among qepA alleles.

Methods

A translational qepA1-yfp fusion under the control of the qepA1 upstream region was cloned into the Escherichia coli chromosome. Expression of the fusion protein was measured in the presence of various antibiotics. Deletions within the upstream region were introduced to identify regions involved in gene expres

SUBMITTER: Brandis G 

PROVIDER: S-EPMC8120332 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature