Alterations in the Duodenal Fluid Microbiome of Patients With Pancreatic Cancer.

ABSTRACT:

Background & aims

The tumor microbiome of patients with pancreas ductal adenocarcinoma (PDAC) includes bacteria normally present in the upper gastrointestinal tract. If the predominant source of intratumoral bacteria in patients with PDAC is retrograde migration from the duodenum, duodenal fluid could be a representative biospecimen for determining microbiome profiles of patients with PDAC or at risk of developing PDAC.

Methods

We performed a case-control study comparing bacterial and fungal (16S and 18S rRNA) profiles of secretin-stimulated duodenal fluid collections from 308 patients undergoing duodenal endoscopy including 134 normal pancreas control subjects, 98 patients with pancreatic cyst(s) and 74 patients with PDAC.

Results

Alterations in duodenal fluid microb

SUBMITTER: Kohi S 

PROVIDER: S-EPMC8120597 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature