Quantifying persistence in the T-cell signaling network using an optically controllable antigen receptor.

ABSTRACT: T cells discriminate between healthy and infected cells with remarkable sensitivity when mounting an immune response, which is hypothesized to depend on T cells combining stimuli from multiple antigen-presenting cell interactions into a more potent response. To quantify the capacity for T cells to accomplish this, we have developed an antigen receptor that is optically tunable within cell conjugates, providing control over the duration, and intensity of intracellular T-cell signaling. We observe limited persistence within the T-cell intracellular network on disruption of receptor input, with signals dissipating entirely in ~15 min, and directly show sustained proximal receptor signaling is required to maintain gene transcription. T cells thus primarily accumulate the outputs of gene expres

SUBMITTER: Harris MJ 

PROVIDER: S-EPMC8120804 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature