Combining transposon mutagenesis and reporter genes to identify novel regulators of the topA promoter in Streptomyces.

ABSTRACT:

Background

Identifying the regulatory factors that control transcriptional activity is a major challenge of gene expression studies. Here, we describe the application of a novel approach for in vivo identification of regulatory proteins that may directly or indirectly control the transcription of a promoter of interest in Streptomyces.

Results

A method based on the combination of Tn5 minitransposon-driven random mutagenesis and lux reporter genes was applied for the first time for the Streptomyces genus. As a proof of concept, we studied the topA supercoiling-sensitive promoter, whose activity is dependent on unknown regulatory factors. We found that the sco4804 gene product positively influences topA transcription in S. coelicolor, demonstrating SCO4804 as a novel player in

SUBMITTER: Gongerowska-Jac M 

PROVIDER: S-EPMC8120823 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature