Quantifying splice-site usage: a simple yet powerful approach to analyze splicing.

ABSTRACT: RNA splicing, and variations in this process referred to as alternative splicing, are critical aspects of gene regulation in eukaryotes. From environmental responses in plants to being a primary link between genetic variation and disease in humans, splicing differences confer extensive phenotypic changes across diverse organisms (1-3). Regulation of splicing occurs through differential selection of splice sites in a splicing reaction, which results in variation in the abundance of isoforms and/or splicing events. However, genomic determinants that influence splice-site selection remain largely unknown. While traditional approaches for analyzing splicing rely on quantifying variant transcripts (i.e. isoforms) or splicing events (i.e. intron retention, exon skipping etc.) (4), recent approac

SUBMITTER: Dent CI 

PROVIDER: S-EPMC8121094 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature