Brain endothelial tricellular junctions as novel sites for T cell diapedesis across the blood-brain barrier.

ABSTRACT: The migration of activated T cells across the blood-brain barrier (BBB) is a critical step in central nervous system (CNS) immune surveillance and inflammation. Whereas T cell diapedesis across the intact BBB seems to occur preferentially through the BBB cellular junctions, impaired BBB integrity during neuroinflammation is accompanied by increased transcellular T cell diapedesis. The underlying mechanisms directing T cells to paracellular versus transcellular sites of diapedesis across the BBB remain to be explored. By combining in vitro live-cell imaging of T cell migration across primary mouse brain microvascular endothelial cells (pMBMECs) under physiological flow with serial block-face scanning electron microscopy (SBF-SEM), we have identified BBB tricellular junctions as novel sites

SUBMITTER: Castro Dias M 

PROVIDER: S-EPMC8121105 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature