Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy.
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ABSTRACT:
SUBMITTER: Lu Y
PROVIDER: S-EPMC8121772 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Publications
Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy.
Lu Yan Y Wang Fangfang F Wang Haoyu H Bastians Philipp P Hua Yunfeng Y
STAR protocols 20210504 2
This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, <i>en bloc</i> staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-scale, nanometer-resolution three-dimensional imaging of subcellular structures in a targeted tonotopic range of the cochlea and enables fast volumetric scan at submicron resolution using a compact X-ra ...[more]
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