Biofilm viability checker: An open-source tool for automated biofilm viability analysis from confocal microscopy images.

ABSTRACT: Quantifying biofilm formation on surfaces is challenging because traditional microbiological methods, such as total colony-forming units (CFUs), often rely on manual counting. These are laborious, resource intensive techniques, more susceptible to human error. Confocal laser scanning microscopy (CLSM) is a high-resolution technique that allows 3D visualisation of biofilm architecture. In combination with a live/dead stain, it can be used to quantify biofilm viability on both transparent and opaque surfaces. However, there is little consensus on the appropriate methodology to apply in confocal micrograph processing. In this study, we report the development of an image analysis approach to repeatably quantify biofilm viability and surface coverage. We also demonstrate its use for a range of

SUBMITTER: Mountcastle SE 

PROVIDER: S-EPMC8121819 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature