Comparison of gene expression and biotransformation activity of HepaRG cells under static and dynamic culture conditions.

ABSTRACT: Flow conditions have been shown to be important in improving longevity and functionality of primary hepatocytes, but the impact of flow on HepaRG cells is largely unknown. We studied the expression of genes encoding CYP enzymes and transporter proteins and CYP1 and CYP3A4 activity during 8 weeks of culture in HepaRG cells cultured under static conditions (conventional 24-/96-well plate culture with common bicarbonate/CO₂ buffering) and under flow conditions in an organ-on-chip (OOC) device. Since the OOC-device is a closed system, bicarbonate/CO₂ buffering was not possible, requiring application of another buffering agent, such as HEPES. In order to disentangle the effects of HEPES from the effects of flow, we also applied HEPES-supplemented medium in static cultures

SUBMITTER: Duivenvoorde LPM 

PROVIDER: S-EPMC8121841 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature