Single-cell RNA sequencing of human liver reveals hepatic stellate cell heterogeneity.

ABSTRACT:

Background & aims

The multiple vital functions of the human liver are performed by highly specialised parenchymal and non-parenchymal cells organised in complex collaborative sinusoidal units. Although crucial for homeostasis, the cellular make-up of the human liver remains to be fully elucidated. Here, single-cell RNA-sequencing was used to unravel the heterogeneity of human liver cells, in particular of hepatocytes (HEPs) and hepatic stellate cells (HSCs).

Method

The transcriptome of ~25,000 freshly isolated human liver cells was profiled using droplet-based RNA-sequencing. Recently published data sets and RNA in situ hybridisation were integrated to validate and locate newly identified cell populations.

Results

In total, 22 cell populations were annotated th

SUBMITTER: Payen VL 

PROVIDER: S-EPMC8121977 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature