Transcriptome profiling of insulin sensitive tissues from GH deficient mice following GH treatment.

ABSTRACT:

Purpose

Most studies that have examined the transcriptional response to GH have been performed with a single tissue. Thus, the current study performed RNASeq across three insulin-sensitive tissues of GH-treated GH deficient (GHKO) mice.

Methods

GHKO mice were injected with recombinant human GH (hGH) or vehicle daily for 5 days and adipose, liver, and muscle tissues were collected 4 h after the final injection. RNA was isolated from the tissues and sequenced. Genes that were differentially expressed between GH and vehicle treatments were further analyzed. Enrichment analysis and topology-aware pathway analysis were performed.

Results

GHKO mice treated with hGH had expected phenotypic alterations, with increased body, fat, fluid, liver, and muscle mass, and increased se

SUBMITTER: Young JA 

PROVIDER: S-EPMC8122029 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature