Advantage of 16S rRNA amplicon sequencing in Helicobacter pylori diagnosis.

ABSTRACT:

Background

16S rRNA amplicon sequencing is an accurate method of detecting microbial infection without culture. It is unclear if sequencing has additional benefits over routine diagnostic methods for Helicobacter pylori testing.

Methods

We enrolled Mongolian volunteers with dyspepsia. Using routine diagnostic methods, positive H. pylori was defined as positive results on histology/immunohistochemistry, culture, rapid urease test, or serology; negative H. pylori was defined by negative results from all these tests. We performed 16S rRNA sequencing on gastric biopsy specimens and calculated cutoffs for operational taxonomic units (OTUs) and relative abundance (RA) to define positive results using ROC curves.

Results

We examined 161 individuals with a mean age of 43.6 ye

SUBMITTER: Gantuya B 

PROVIDER: S-EPMC8122063 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature