Standard procedures for native CZE-MS of proteins and protein complexes up to 800 kDa.

ABSTRACT: Native mass spectrometry (nMS) is a rapidly growing method for the characterization of large proteins and protein complexes, preserving "native" non-covalent inter- and intramolecular interactions. Direct infusion of purified analytes into a mass spectrometer represents the standard approach for conducting nMS experiments. Alternatively, CZE can be performed under native conditions, providing high separation performance while consuming trace amounts of sample material. Here, we provide standard operating procedures for acquiring high-quality data using CZE in native mode coupled online to various Orbitrap mass spectrometers via a commercial sheathless interface, covering a wide range of analytes from 30-800 kDa. Using a standard protein mix, the influence of various CZE method parameters w

SUBMITTER: Jooß K 

PROVIDER: S-EPMC8122066 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature