RGB-Marking to Identify Patterns of Selection and Neutral Evolution in Human Osteosarcoma Models.

ABSTRACT: Osteosarcoma (OS) is a highly aggressive tumor characterized by malignant cells producing pathologic bone; the disease presents a natural tendency to metastasize. Genetic studies indicate that the OS genome is extremely complex, presenting signs of macro-evolution, and linear and branched patterns of clonal development. However, those studies were based on the phylogenetic reconstruction of next-generation sequencing (NGS) data, which present important limitations. Thus, testing clonal evolution in experimental models could be useful for validating this hypothesis. In the present study, lentiviral LeGO-vectors were employed to generate colorimetric red, green, blue (RGB)-marking in murine, canine, and human OS. With this strategy, we studied tumor heterogeneity and the clonal dynamics occu

SUBMITTER: Gambera S 

PROVIDER: S-EPMC8122697 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature