Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays.

ABSTRACT: Background: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. Methods: The cellular antioxidant assay (CAA) assay was employed for direct scavenging assays. In the indirect approach, the influence of each test substance on the gene and protein expression and activity of selected antioxidant enzymes was observed. One assay also dealt with activation of the Nrf2-ARE pathway. The overall effect of each compound was measured using a glucose oxidative stress protection assay. Results: Among the test compounds, acteoside showed the highest direct scavenging activity and no effect on the expression of antioxidant enzymes. It i

SUBMITTER: Treml J 

PROVIDER: S-EPMC8123583 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature