Concordance and characterization of massively parallel sequencing at 58 STRs in a Tibetan population.

ABSTRACT:

Background

Massively parallel sequencing (MPS) is a promising supplementary method for forensic casework in short tandem repeats (STRs) genotyping, owing to several advantageous features in comparison to traditional capillary electrophoresis (CE). However, the application of MPS in casework requires accessible datasets from the worldwide population to enrich the allele frequencies of sequence-based STR genotypes.

Methods

In this study, we report the characterization of sequence-based allele frequencies of 58 STRs from a Tibetan population comprising 120 unrelated individuals using the ForenSeq™ DNA Signature Prep Kit. A concordance study evaluating MPS and CE allele data was performed to ensure that MPS is compatible with current CE-based forensic databases. The diversity of

SUBMITTER: Li H 

PROVIDER: S-EPMC8123751 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature