Characterization of Extracellular Vesicle Cargo in Sjogren's Syndrome through a SWATH-MS Proteomics Approach.

ABSTRACT: Primary Sjögren's syndrome (pSS) is a complex heterogeneous disease characterized by a wide spectrum of glandular and extra-glandular manifestations. In this pilot study, a SWATH-MS approach was used to monitor extracellular vesicles-enriched saliva (EVs) sub-proteome in pSS patients, to compare it with whole saliva (WS) proteome, and assess differential expressed proteins between pSS and healthy control EVs samples. Comparison between EVs and WS led to the characterization of compartment-specific proteins with a moderate degree of overlap. A total of 290 proteins were identified and quantified in EVs from healthy and pSS patients. Among those, 121 proteins were found to be differentially expressed in pSS, 82% were found to be upregulated, and 18% downregulated in pSS samples. The most rep

SUBMITTER: Finamore F 

PROVIDER: S-EPMC8124455 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature