Dataset Information

Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation.

ABSTRACT: Mounting evidence indicates the key role of nitrogen (N) on diverse processes in plant, including development and defense. Using a combined transcriptomics and metabolomics approach, we studied the response of seedlings to N starvation of two different tetraploid wheat genotypes from the two main domesticated subspecies: emmer and durum wheat. We found that durum wheat exhibits broader and stronger response in comparison to emmer as seen from the expression pattern of both genes and metabolites and gene enrichment analysis. They showed major differences in the responses to N starvation for transcription factor families, emmer showed differential reduction in the levels of primary metabolites while durum wheat exhibited increased levels of most of them to N starvation. The correlation-based networks, including the differentially expressed genes and metabolites, revealed tighter regulation of metabolism in durum wheat in comparison to emmer. We also found that glutamate and γ-aminobutyric acid (GABA) had highest values of centrality in the metabolic correlation network, suggesting their critical role in the genotype-specific response to N starvation of emmer and durum wheat, respectively. Moreover, this finding indicates that there might be contrasting strategies associated to GABA and glutamate signaling modulating shoot vs. root growth in the two different wheat subspecies.

SUBMITTER: Beleggia R

PROVIDER: S-EPMC8124848 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation.

Beleggia Romina R Omranian Nooshin N Holtz Yan Y Gioia Tania T Fiorani Fabio F Nigro Franca M FM Pecchioni Nicola N De Vita Pasquale P Schurr Ulrich U David Jacques L JL Nikoloski Zoran Z Papa Roberto R

International journal of molecular sciences 20210430 9

Mounting evidence indicates the key role of nitrogen (N) on diverse processes in plant, including development and defense. Using a combined transcriptomics and metabolomics approach, we studied the response of seedlings to N starvation of two different tetraploid wheat genotypes from the two main domesticated subspecies: emmer and durum wheat. We found that durum wheat exhibits broader and stronger response in comparison to emmer as seen from the expression pattern of both genes and metabolites ...[more]

PMID: 33946478

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Project description:High temperatures pose a major threat to wheat productivity and necessitate the development of new cultivars that are resilient to future heat stress. Wild emmer (Triticum turgidum L. ssp. dicoccoides), which is a direct progenitor of domesticated durum wheat (Triticum turgidum L. ssp. durum) and contributor to the A and B genome of bread wheat (Triticum aestivum), offers a valuable genetic reservoir for developing climate-resilient wheat. However, the morphology of wild emmer is different from that of durum and bread wheat, in particular, the spikelets are fragile and naturally fall off, making it difficult to study its agronomic traits. In this study, we created nine backcrossed families between the popular durum wheat cultivar 'Miki 3' and nine wild emmer accessions collected from northern and southern lineages of this species. The objective was to investigate the intraspecific genetic variation in wild emmer and identify traits associated with heat stress tolerance. We evaluated these nine families under multi-environments ranging from optimum to severe heat stress conditions in Japan and Sudan and measured important agronomic traits. The result showed that two families, developed from accessions of both northern and southern lineages exhibited high harvest index, elevated chlorophyll content, and reduced canopy temperature under heat stress. Additionally, one family developed from an accession of the southern lineage displayed high biomass, harvest index, and seed number under heat-stress conditions. These three families produced high heat tolerant lines with unique introgressed segments from their wild emmer parents on chromosomes 1A, 2B, 5B, 6B, and 7B, which may be linked to heat resilience. From these results, we were able to identify significant intraspecific diversity between the wild emmer accessions in terms of heat stress tolerance. However, no significant tendency between the northern and southern lineages of wild emmer has been identified. These findings emphasize the need to harness not only the interspecific but also the intraspecific genetic variation of wild emmer diversity to uncover valuable genes for heat stress tolerance in wheat breeding programs.

Dataset Information

Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation.

Publications

Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation.

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