MiR-183-5p Is a Potential Molecular Marker of Systemic Lupus Erythematosus.

ABSTRACT:

Objective

To investigate microRNA (miRNA) expression profiles in individuals with systemic lupus erythematosus (SLE) and identify the valuable miRNA biomarkers in diagnosing and monitoring SLE.

Methods

Next-generation sequencing (NGS) was performed to assess miRNA amounts in peripheral blood mononuclear cells (PBMCs) from four SLE cases and four healthy controls. Quantitative polymerase chain reaction (qPCR) was carried out for validating candidate miRNAs in 32 SLE cases and 32 healthy controls. In addition, receiver operating characteristic (ROC) curve analysis was completed to evaluate diagnostic performance. Finally, the associations of candidate miRNAs with various characteristics of SLE were analyzed.

Results

A total of 157 miRNAs were upregulated, and 110 miRNAs

SUBMITTER: Zhou S 

PROVIDER: S-EPMC8124875 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature