Impact of uORFs in mediating regulation of translation in stress conditions.

ABSTRACT:

Background

A large fraction of genes contains upstream ORFs (uORFs) in the 5' untranslated region (5'UTR). The translation of uORFs can inhibit the translation of the main coding sequence, for example by causing premature dissociation of the two ribosomal units or ribosome stalling. However, it is currently unknown if most uORFs are inhibitory or if this activity is restricted to specific cases. Here we interrogate ribosome profiling data from three different stress experiments in yeast to gain novel insights into this question.

Results

By comparing ribosome occupancies in different conditions and experiments we obtain strong evidence that, in comparison to primary coding sequences (CDS), which undergo translational arrest during stress, the translation of uORFs is mostly un

SUBMITTER: Moro SG 

PROVIDER: S-EPMC8126119 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature