LAMA3 DNA methylation and transcriptome changes associated with chemotherapy resistance in ovarian cancer.

ABSTRACT:

Objective

LAMA3 is a widely studied methylated gene in multiple tumors, but the relationship between chemotherapy resistance in ovarian cancer is unclear. In this study, LAMA3 methylation was predicted by bioinformatics, and the ability of LAMA3 methylation to predict the chemotherapy resistance and prognosis of ovarian cancer was confirmed in experiments.

Methods

Multiple databases have performed the bioinformatics analysis of methylation and transcription factor binding site (TFBS) on the promoter region of LAMA3 gene. Pyrosequencing detected the methylation of LAMA3. QRT-PCR and immunohistochemistry detected the expression of LAMA3. Real Time Cell Analyzer (RTCA) detects changes in cell proliferation, migration and invasion ability. Flow cytometry was used to detect apopt

SUBMITTER: Feng LY 

PROVIDER: S-EPMC8126133 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature