Human Engineered Heart Tissue Patches Remuscularize the Injured Heart in a Dose-Dependent Manner.

ABSTRACT:

Background

Human engineered heart tissue (EHT) transplantation represents a potential regenerative strategy for patients with heart failure and has been successful in preclinical models. Clinical application requires upscaling, adaptation to good manufacturing practices, and determination of the effective dose.

Methods

Cardiomyocytes were differentiated from 3 different human induced pluripotent stem cell lines including one reprogrammed under good manufacturing practice conditions. Protocols for human induced pluripotent stem cell expansion, cardiomyocyte differentiation, and EHT generation were adapted to substances available in good manufacturing practice quality. EHT geometry was modified to generate patches suitable for transplantation in a small-animal model and perspe

SUBMITTER: Querdel E 

PROVIDER: S-EPMC8126500 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature