Polyhydroxybutyrate (PHB) Production Using an Arabinose-Inducible Expression System in Comparison With Cold Shock Inducible Expression System in Escherichia coli.

ABSTRACT: Cupriavidus necator strain A-04 has shown 16S rRNA gene identity to the well-known industrial strain C. necator H16. Nevertheless, the cell characteristics and polyhydroxyalkanoate (PHA) production ability of C. necator strain A-04 were different from those of C. necator H16. This study aimed to express PHA biosynthesis genes of C. necator strain A-04 in Escherichia coli via an arabinose-inducible expression system. In this study, the PHA biosynthesis operon of C. necator strain A-04, consisting of three genes encoding acetyl-CoA acetyltransferase (phaA _A-04, 1182 bp, 40.6 kDa), acetoacetyl-CoA reductase (phaB _A-04, 741 bp, 26.4 kDa) and PHB synthase Class I (phaC _A-04, 1770 bp), was iden

SUBMITTER: Napathorn SC 

PROVIDER: S-EPMC8126650 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature