Association of <i>Fusobacterium nucleatum</i> with Specific T-cell Subsets in the Colorectal Carcinoma Microenvironment.

ABSTRACT:

Purpose

While evidence indicates that Fusobacterium nucleatum (F. nucleatum) may promote colorectal carcinogenesis through its suppressive effect on T-cell-mediated antitumor immunity, the specific T-cell subsets involved remain uncertain.

Experimental design

We measured F. nucleatum DNA within tumor tissue by quantitative PCR on 933 cases (including 128 F. nucleatum-positive cases) among 4,465 incident colorectal carcinoma cases in two prospective cohorts. Multiplex immunofluorescence combined with digital image analysis and machine learning algorithms for CD3, CD4, CD8, CD45RO (PTPRC isoform), and FOXP3 measured various T-cell subsets. We leveraged data on Bifidobacterium, microsatellite instability (MSI), tumor whole-exome sequencing, an

SUBMITTER: Borowsky J 

PROVIDER: S-EPMC8127352 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature