Establishment and characterization of murine models of asthma and subcutaneous immunotherapy for Humulus pollen allergy.

ABSTRACT:

Introduction

Humulus pollen is an important cause of allergic asthma in East Asia. There have been some murine models for Humulus pollen allergy established by intraperitoneal (IP) sensitization and nasal drip stimulation, but they were not comprehensive enough. Here, we used atomized inhalation for challenge and compared the subcutaneous (SC) and IP sensitization routes to determine the optimal method to establish a model of asthma induced by Humulus pollen. Subsequently, we tried to develop a rapid subcutaneous immunotherapy (SCIT) model for Humulus allergy.

Methods

BALB/c Mice were sensitized through the SC or IP route, with respective reference to previously established sensitization methods and allergen dosing, and challenged with nebulized Humulus pollen extract to ind

SUBMITTER: Xi GP 

PROVIDER: S-EPMC8127558 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature