Cathepsin K maintains the compartment of bone marrow T lymphocytes in vivo.

ABSTRACT: In this study, we investigated the influence of the loss of cathepsin K (Ctsk) gene on the hematopoietic system in vitro and in vivo. We found that cultures with lineage^- SCA1⁺ KIT⁺ (LSK) cells on Ctsk deficient stromal cells display reduced colony formation and proliferation, with increased differentiation, giving rise to repopulating cells with reduced ability to repopulate the donor LSKs and T cell compartments in the bone marrow (BM). Subsequent in vivo experiments showed impairment of lymphocyte numbers, but, gross effects on early hematopoiesis or myelopoiesis were not found. Most consistently in in vivo experimental settings, we found a significant reduction of (donor) T cell numbers in the BM. Lymphocyte deregulation is also found in transplantation

SUBMITTER: Hausinger R 

PROVIDER: S-EPMC8127559 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature